2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Abubuwan da aka saba amfani da su a cikin lanƙwasa na daidaitawar rarrabawar ƙwayoyin halitta: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Kayan aiki da kayan aiki
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Gabaɗaya, adadin amino acid a cikin kayayyakin Sustar ya fi na kayayyakin Zinpro girma.
Sashe na 8 Tasirin amfani
Tasirin ma'adanai daban-daban akan aikin samarwa da ingancin ƙwai na kaji a lokacin kwanciya
Tsarin Samarwa
Fasahar chelation mai niyya
Fasahar fitar da shear emulsification
Fasahar feshi da busarwa ta matsi
Fasahar sanyaya da kuma rage danshi
Fasaha mai zurfi ta sarrafa muhalli
Shafi na A: Hanyoyi don Tabbatar da rarrabawar ƙwayoyin peptides
Yarda da ma'auni: GB/T 22492-2008
1 Ka'idar Gwaji:
An tantance shi ta hanyar amfani da sinadarin gel mai inganci. Wato, ta amfani da sinadarin mai ramuka a matsayin lokaci mai tsayi, bisa ga bambancin girman sinadarin kwayoyin halitta na samfurin da aka samo don rabuwa, wanda aka gano a cikin haɗin peptide na tsawon sha na ultraviolet na 220nm, ta amfani da software na sarrafa bayanai na musamman don tantance rarrabawar ƙwayoyin halitta ta hanyar amfani da sinadarin gel filtering chromatography (watau, software na GPC), an sarrafa chromatograms da bayanan su, an ƙididdige su don samun girman nauyin sinadarin waken soya da kewayon rarrabawa.
2. Masu sake haɗawa
Ruwan gwaji ya kamata ya cika ƙa'idodin ruwan sakandare a cikin GB/T6682, amfani da abubuwan maye, banda tanadi na musamman, tsarkakakke ne a fannin nazari.
2.1 Abubuwan da ke haifar da amsawa sun haɗa da acetonitrile (tsaftace a fannin chromatographic), trifluoroacetic acid (tsaftace a fannin chromatographic),
2.2 Abubuwan da aka saba amfani da su a cikin lanƙwasa na daidaitawar rarrabawar ƙwayoyin halitta: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Kayan aiki da kayan aiki
3.1 Babban Aikin Gilashin Ruwan Aiki (HPLC): wurin aiki ko mai haɗa bayanai na chromatographic tare da na'urar gano UV da software na sarrafa bayanai na GPC.
3.2 Na'urar tacewa da cire iskar gas ta hanyar amfani da injinan tacewa na zamani.
3.3 Ma'aunin lantarki: ƙimar digiri 0.000 1g.
Matakai 4 na aiki
4.1 Yanayin Chromatographic da gwaje-gwajen daidaitawa da tsarin (yanayin tunani)
- 4.1.1 Ginshiƙin Chromatographic: TSKgelG2000swxl300 mm×7.8 mm (diamita na ciki) ko wasu ginshiƙan gel iri ɗaya waɗanda ke da irin wannan aikin da ya dace da tantance sunadaran da peptides.
- 4.1.2 Matakin Wayar Hannu: Acetonitrile + ruwa + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Tsawon tsayin ganowa: 220 nm.
- 4.1.4 Yawan kwarara: 0.5 mL/min.
- 4.1.5 Lokacin ganowa: minti 30.
- 4.1.6 Girman allurar samfurin: 20μL.
- 4.1.7 Zafin ginshiƙi: zafin ɗaki.
- 4.1.8 Domin tabbatar da cewa tsarin chromatographic ya cika buƙatun ganowa, an tsara cewa a ƙarƙashin yanayin chromatographic da ke sama, ingancin ginshiƙin gel chromatographic, watau, adadin faranti na ka'ida (N), bai gaza 10000 da aka ƙididdige bisa ga kololuwar ma'aunin tripeptide (Glycine-Glycine-Glycine) ba.
- 4.2 Samar da daidaitattun lanƙwasa na nauyin kwayoyin halitta
- An shirya mafita daban-daban na peptide na ƙwayoyin halitta masu yawan taro na 1 mg / mL ta hanyar daidaita yanayin motsi, a gauraya su a wani rabo, sannan a tace su ta hanyar membrane na lokaci na halitta tare da girman ramin 0.2 μm ~ 0.5 μm sannan a saka su cikin samfurin, sannan aka sami chromatograms na ƙa'idodin. An sami lanƙwasa na daidaita nauyin ƙwayoyin halitta da daidaitonsu ta hanyar zana logarithm na nauyin kwayoyin halitta dangane da lokacin riƙewa ko ta hanyar komawa kan layi.
4.3 Maganin samfurin
A auna samfurin 10mg daidai a cikin kwalbar volumetric mai girman 10mL, a ƙara ƙaramin matakin motsi, ana girgiza shi ta hanyar ultrasonic na tsawon minti 10, don samfurin ya narke gaba ɗaya kuma a gauraya, a narkar da shi da matakin motsi zuwa sikelin, sannan a tace ta cikin membrane na lokaci-lokaci na halitta tare da girman rami na 0.2μm ~ 0.5μm, kuma an yi nazarin tacewar bisa ga yanayin chromatographic a cikin A.4.1.
- 5. Lissafin rarrabawar nauyin kwayoyin halitta
- Bayan nazarin maganin samfurin da aka shirya a cikin 4.3 a ƙarƙashin yanayin chromatographic na 4.1, ana iya samun nauyin kwayoyin halitta na samfurin da kewayon rarrabawa ta hanyar maye gurbin bayanan chromatographic na samfurin zuwa cikin lanƙwasa na daidaitawa 4.2 tare da software na sarrafa bayanai na GPC. Ana iya ƙididdige rarrabawar tarin kwayoyin halitta na peptides daban-daban ta hanyar hanyar daidaita yankin kololuwa, bisa ga dabarar: X=A/A jimilla×100
- A cikin dabarar: X - Kashi na taro na peptide mai nauyin kwayoyin halitta a cikin jimlar peptide a cikin samfurin, %;
- A - Yankin kololuwar peptide na ƙwayoyin halitta mai kama da juna;
- Jimilla A - jimlar yankunan kololuwar kowace peptide mai nauyin kwayoyin halitta, wanda aka ƙididdige zuwa wuri ɗaya na goma.
- 6 Maimaitawa
- Bambancin da ke tsakanin ƙayyadaddun bayanai guda biyu masu zaman kansu da aka samu a ƙarƙashin sharuɗɗan maimaitawa ba zai wuce kashi 15% na matsakaicin lissafi na ƙayyadaddun bayanai guda biyu ba.
- Shafi na B: Hanyoyin Tabbatar da Amino Acid Marasa Kyau
- Yarda da ma'auni: Q/320205 KAVN05-2016
- 1.2 Masu sake haɗawa da kayan aiki
- Glacial acetic acid: tsantsar a fannin nazari
- Sinadarin Perchloric: 0.0500 mol/L
- Alamar: 0.1% mai nuna launin lu'ulu'u mai haske (glacial acetic acid)
- 2. Tabbatar da amino acid kyauta
An busar da samfuran a zafin digiri 80 na Celsius na tsawon awa 1.
Sanya samfurin a cikin busasshen akwati don ya huce zuwa zafin ɗaki ko kuma ya huce zuwa zafin da za a iya amfani da shi.A auna kimanin gram 0.1 na samfurin (daidai har zuwa gram 0.001) a cikin busasshen kwalba mai siffar mazugi mai nauyin 250 mL.Ci gaba da sauri zuwa mataki na gaba don guje wa samfurin shan danshi na yanayiSai a zuba 25 ml na glacial acetic acid a gauraya sosai ba fiye da minti 5 ba.Ƙara digo biyu na alamar lu'ulu'u mai launin crystalA tace sinadarin perchloric acid da sinadarin 0.0500 mol / L (±0.001) na sinadarin titration har sai ruwan ya canza daga launin shunayya zuwa ƙarshensa.
Yi rikodin adadin maganin da aka yi amfani da shi.
- Yi gwajin da babu komai a lokaci guda.
- 3. Lissafi da sakamako
- Ana bayyana sinadarin amino acid kyauta X a cikin reagent a matsayin juzu'in taro (%) kuma ana ƙididdige shi bisa ga dabarar: X = C × (V1-V0) × 0.1445/M × 100%, a cikin dabarar tne:
- C - Tattara sinadarin perchloric acid na yau da kullun a cikin moles a kowace lita (mol/L)
- V1 - Ƙarar da ake amfani da ita don yin titration na samfurori tare da maganin perchloric acid na yau da kullun, a cikin milliliters (mL).
- Vo - Ƙarar da ake amfani da ita don titration blank tare da maganin perchloric acid na yau da kullun, a cikin milliliters (mL);
M - Nauyin samfurin, a cikin gram (g).
| 0.1445: Matsakaicin nauyin amino acid wanda ya yi daidai da 1.00 mL na maganin perchloric acid na yau da kullun [c (HClO4) = 1.000 mol / L]. | 4.2.3 Maganin titration na Cerium sulfate na yau da kullun: yawan c [Ce (SO4) 2] = 0.1 mol/L, wanda aka shirya bisa ga GB/T601. | |
| Yarda da ƙa'idodi: Q/70920556 71-2024 | 1. Ka'idar ƙaddara (Fe a matsayin misali) | Hadaddun ƙarfe na amino acid suna da ƙarancin narkewa a cikin ethanol mai narkewa kuma ions na ƙarfe kyauta suna narkewa a cikin ethanol mai narkewa, an yi amfani da bambancin narkewa tsakanin su biyu a cikin ethanol mai narkewa don tantance ƙimar chelation na hadaddun ƙarfe na amino acid. |
| A cikin dabarar: V1 - ƙarar maganin cerium sulfate na yau da kullun da aka yi amfani da shi don titration na maganin gwaji, mL; | Ethanol mai ruwa; sauran iri ɗaya ne da sashe na 4.5.2 a cikin GB/T 27983-2011. | 3. Matakan nazari |
| Yi gwaji guda biyu a layi ɗaya. A auna 0.1g na samfurin a busar da shi a 103±2℃ na tsawon awa 1, daidai da 0.0001g, a ƙara 100mL na ethanol mai ruwa don narkewa, a tace, a tace ragowar da aka wanke da 100mL na ethanol mai ruwa don akalla sau uku, sannan a mayar da ragowar zuwa cikin kwalbar conical mai 250mL, a ƙara 10mL na maganin sulfuric acid bisa ga sashe na 4.5.3 a cikin GB/T27983-2011, sannan a yi waɗannan matakan bisa ga sashe na 4.5.3 "Zafi don narkewa sannan a bar shi ya huce" a cikin GB/T27983-2011. A yi gwajin da babu komai a lokaci guda. | 4. Tantance jimillar adadin baƙin ƙarfe | 4.1 Ka'idar tantancewa iri ɗaya ce da sashe na 4.4.1 a cikin GB/T 21996-2008. |
4.2. Ma'aikatan sake sarrafawa da mafita
| 4.2.1 Haɗaɗɗen acid: A zuba 150mL na sulfuric acid da 150mL na phosphoric acid a cikin 700mL na ruwa a gauraya sosai. | 4.2.2 Maganin sinadarin sodium diphenylamine sulfonate: 5g/L, wanda aka shirya bisa ga GB/T603. | 4.2.3 Maganin titration na Cerium sulfate na yau da kullun: yawan c [Ce (SO4) 2] = 0.1 mol/L, wanda aka shirya bisa ga GB/T601. | |
| 4.3 Matakan nazari | Yi gwaji guda biyu a layi ɗaya. A auna 0.1g na samfurin, daidai yake da 020001g, a saka a cikin kwalbar mazugi mai girman 250mL, a ƙara 10mL na gaurayen acid, bayan an narkar da shi, a ƙara 30ml na ruwa da digo 4 na maganin sodium dianiline sulfonate, sannan a yi waɗannan matakan bisa ga sashe na 4.4.2 a cikin GB/T21996-2008. A yi gwajin da babu komai a lokaci guda. | 4.4 Wakiltar sakamako | An ƙididdige jimlar adadin ƙarfe X1 na hadaddun ƙarfe na amino acid dangane da rabon taro na ƙarfe, ƙimar da aka bayyana a cikin %, bisa ga dabarar (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - maganin cerium sulfate na yau da kullun da ake amfani da shi don titration na maganin mara komai, mL; | V0 - maganin cerium sulfate na yau da kullun da ake amfani da shi don titration na maganin mara komai, mL; | C - Ainihin yawan maganin cerium sulfate na yau da kullun, mol/L5. Lissafin adadin ƙarfe a cikin chelatesAn ƙididdige yawan ƙarfe X2 a cikin chelate dangane da yawan ƙarfe, ƙimar da aka bayyana a cikin %, bisa ga dabarar: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| A cikin dabarar: V1 - ƙarar maganin cerium sulfate na yau da kullun da aka yi amfani da shi don titration na maganin gwaji, mL; | V2 - maganin cerium sulfate na yau da kullun da ake amfani da shi don titration na maganin da babu komai, mL;nom1-Matsakaicin samfurin, g. Ɗauki matsakaicin lissafi na sakamakon tantancewa mai layi ɗaya a matsayin sakamakon tantancewa, kuma cikakken bambancin sakamakon tantancewa mai layi ɗaya bai wuce 0.3% ba. | 0.05585 - nauyin ƙarfe mai ƙarfe da aka bayyana a cikin gram daidai da 1.00 mL na maganin cerium sulfate na yau da kullun C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Matsakaicin samfurin, g. Ɗauki matsakaicin lissafi na sakamakon tantancewa mai layi ɗaya a matsayin sakamakon tantancewa, kuma cikakken bambancin sakamakon tantancewa mai layi ɗaya bai wuce 0.3% ba. | 6. Lissafin ƙimar chelationMatsakaicin Chelation X3, ƙimar da aka bayyana a cikin %, X3 = X2/X1 × 100Shafi na C: Hanyoyin da za a bi don tantance yawan sinadarin Zinpro |
Yarda da ma'auni: Q/320205 KAVNO7-2016
1. Masu sake haɗawa da kayan aiki
a) Glacial acetic acid: tsantsar nazari; b) Perchloric acid: 0.0500mol/L; c) Alamar: 0.1% alamar lu'ulu'u mai launin crystal (glacial acetic acid)
2. Tabbatar da amino acid kyauta
2.1 An busar da samfuran a zafin 80°C na tsawon awa 1.
2.2 Sanya samfurin a cikin akwati busasshe don ya huce ta halitta zuwa zafin ɗaki ko kuma ya huce zuwa zafin da za a iya amfani da shi.
2.3 A auna kimanin 0.1 g na samfurin (daidai har zuwa 0.001 g) a cikin busasshen kwalba mai siffar mazugi mai nauyin 250 mL
2.4 Ci gaba da sauri zuwa mataki na gaba don guje wa samfurin shan danshi na yanayi.
2.5 A zuba 25mL na glacial acetic acid a gauraya sosai ba fiye da minti 5 ba.
2.6 Ƙara digo biyu na alamar lu'ulu'u mai launin crystal.
2.7 A tace sinadarin perchloric acid da sinadarin 0.0500mol/L (±0.001) na sinadarin titration har sai maganin ya canza daga shunayya zuwa kore na tsawon mintuna 15 ba tare da canza launi a matsayin wurin ƙarshe ba.
2.8 Yi rikodin adadin maganin da aka yi amfani da shi.
2.9 Yi gwajin da babu komai a lokaci guda.
- 3. Lissafi da sakamako
- Catalan
- Physicochemical parameters
V1 - Ƙarar da ake amfani da ita don yin titration na samfurori tare da maganin perchloric acid na yau da kullun, a cikin milliliters (mL).
Vo - Ƙarar da ake amfani da ita don titration blank tare da maganin perchloric acid na yau da kullun, a cikin milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adireshi: Lamba 147 Titin Qingpu, Garin Shouan, Gundumar Pujiang, Birnin Chengdu, Lardin Sichuan, China
Waya: 86-18880477902
Kayayyaki
Ma'adanai marasa tsari
- Ma'adanai masu alamar halitta
- Sawahili
- Sabis na musamman
- Hanyoyin haɗi masu sauri
Bayanin Kamfani
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Danna don bincike | © Haƙƙin mallaka - 2010-2025: An kiyaye dukkan haƙƙoƙi. | Taswirar Yanar Gizo BINCIKE MAFI GIRMA Waya |
| Tel | 86-18880477902 | Javanisanci | Imel |
| 8618880477902 | 'Yan China | Faransanci | |
| Bird | 'Yan China | Faransanci | Jamusanci Sifaniyanci |
| Aquatic animals | Jafananci | Koriya | Larabci Girkanci |
| Baturke | Italiyanci | ||
| Ruminant animal g/head day | January 0.75 | Indonesiya 'Yan Afirka Yaren mutanen Sweden |
Yaren mutanen Poland
- Basque
- Catalan
- Physicochemical parameters
Bahindi
Laos
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgaria
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Croatia
'Yan Holland
| Application object | Urdu 'Yan Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haiti | Hausawa | Kinyarwanda Hmong Ɗan ƙasar Hungary |
| Piglets and fattening pigs | Igbo | Javanisanci | Kanada Khmer Kurdawa |
| Kyrgyzstan | Latin | ||
| Bird | 300～400 | 45～60 | Ba'amurke ɗan ƙasar Macedoniya Malay Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Yaren mutanen Norway
- Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbia
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Sawahili
'Yan Tajikistan
Tamil
Telugu
'Yan Thailand
| Application object | Urdu 'Yan Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Yadish | Yarbanci | Zulu | Kinyarwanda Oriya Turkmen |
| 'Yan Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
